Rab23 Monoclonal antibody

• 

  WB analysis of human brain using 60056-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60056-1-Ig (RAB23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60056-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60056-1-Ig (RAB23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 60056-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60056-1-Ig (RAB23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

Rab23 Monoclonal Antibody for WB, ELISA

Cat No. 60056-1-Ig

Clone No.3E12B6

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: human brain tissue, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

60056-1-Ig targets Rab23 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Rab23 fusion protein Ag1588
• Full Name: RAB23, member RAS oncogene family
• Calculated Molecular Weight: 237 aa, 27 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC015021
• Gene Symbol: RAB23
• Gene ID (NCBI): 51715
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9ULC3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab23 is a novel member of large Rab small GTPase family. It plays important roles during embryonic development. The brain-enriched Rab23 is the only Rab protein that is known to have a distinct function during central nervous system development, playing an essential role as a negative regulator of the Sonic Hedgehog (Shh) pathway. RAB23 has recently been identified in mesangial cells (MCs), and could serve as a biomarker that indicates the severity of FSGS ( focal segmental glomerulosclerosis). Mutations in RAB23 may result in ACPS2 ( acrocephalopolysyndactyly type 2).

Publications

Species	Application	Title
human,mouse	WB	Curr Biol; Planar Cell Polarity Effector Proteins Inturned and Fuzzy Form a Rab23 GEF Complex.; Authors - Andreas Gerondopoulos ; KD Validated