Rab23 Polyclonal antibody

• Featured Product
• KD/KO Validated

Rab23 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 11101-1-AP

Synonyms

HSPC137, RAB23, Ras related protein Rab 23

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Validation Data Gallery

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  WB analysis of mouse brain using 11101-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11101-1-AP (RAB23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11101-1-AP

  WB result of RAB23 antibody (11101-1-AP, 1:3000) with si-Control and si-Rab23 transfected HEK293 cells.

• 

  WB analysis of rat brain using 11101-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11101-1-AP (RAB23 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11101-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11101-1-AP (RAB23 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11101-1-AP

  IP result of anti-Rab23 (IP:11101-1-AP, 3ug; Detection:11101-1-AP 1:1000) with mouse brain tissue lysate 7000ug.

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  IHC staining of human liver cancer using 11101-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11101-1-AP (RAB23 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 11101-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11101-1-AP (RAB23 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, HEK-293 cells, human brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 5 publications below

Product Information

11101-1-AP targets Rab23 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Rab23 fusion protein Ag1588
• Full Name: RAB23, member RAS oncogene family
• Calculated Molecular Weight: 237 aa, 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC015021
• Gene Symbol: RAB23
• Gene ID (NCBI): 51715
• RRID: AB_2173784
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab23 is a novel member of large Rab small GTPase family. It plays important roles during embryonic development. The brain-enriched Rab23 is the only Rab protein that is known to have a distinct function during central nervous system development, playing an essential role as a negative regulator of the Sonic Hedgehog (Shh) pathway. RAB23 has recently been identified in mesangial cells (MCs), and could serve as a biomarker that indicates the severity of FSGS ( focal segmental glomerulosclerosis). Mutations in RAB23 may result in ACPS2 ( acrocephalopolysyndactyly type 2).

Publications

Species	Application	Title
human,mouse	WB,IF	Curr Biol; Planar Cell Polarity Effector Proteins Inturned and Fuzzy Form a Rab23 GEF Complex.; Authors - Andreas Gerondopoulos ; KD Validated
mouse	WB,IHC	Elife; RAB23 coordinates early osteogenesis by repressing FGF10-pERK1/2 and GLI1.; Authors - Md Rakibul Hasan
human	WB,IF	Oncotarget; Rab23 promotes squamous cell carcinoma cell migration and invasion via integrin β1/Rac1 pathway.; Authors - Qiang Jian ; KD Validated
human	WB	J Cell Sci; Differential role of Rab proteins in ciliary trafficking: Rab23 regulates smoothened levels.; Authors - Boehlke Christopher C ; KD Validated
human	WB,IHC	J Cancer Res Clin Oncol; MicroRNA-367-3p directly targets RAB23 and inhibits proliferation, migration and invasion of bladder cancer cells and increases cisplatin sensitivity; Authors - Xifeng Wei ; KD Validated
human,mouse	WB,IF	Exp Dermatol; Silencing of Rab23 by siRNA inhibits ultraviolet B-induced melanogenesis via downregulation of PKA/CREB/MITF.; Authors - Xuanjin Wei ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guorong (Verified Customer) (11-22-2022)

Multiple bands distributed at 50 and 75 kDa were observed . Does not represent the performance in other kind of samples

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19

Rab23 Antibody Western Blot validation (1:500 dilution) in ARPE-19 (Cat no:11101-1-AP)