RAB22A Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB22A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 12125-1-AP

Synonyms

Rab 22, RAB22, RAB22A, Ras related protein Rab 22A

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Validation Data Gallery

• 

  WB analysis of COLO 320 using 12125-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12125-1-AP (RAB22A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12125-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12125-1-AP (RAB22A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 12125-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12125-1-AP (RAB22A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12125-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12125-1-AP (RAB22A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 12125-1-AP

  IP result of anti-RAB22A (IP:12125-1-AP, 4ug; Detection:12125-1-AP 1:300) with COLO 320 cells lysate 2560 ug.

• 

  IHC staining of human gliomas using 12125-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12125-1-AP (RAB22A antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: COLO 320 cells, Jurkat cells, BxPC-3 cells, A375 cells
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 17 publications below
• IHC: See 7 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Product Information

12125-1-AP targets RAB22A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, canine, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB22A fusion protein Ag2769
• Full Name: RAB22A, member RAS oncogene family
• Calculated Molecular Weight: 194 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC015710
• Gene Symbol: RAB22A
• Gene ID (NCBI): 57403
• RRID: AB_2173765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB22A is a small GTPase that is expressed ubiquitously in mammalian tissues. It localizes in the MHCI-containing recycling endosomal tubules and functions in endocytic pathway. RAB22A may regulate the dynamic interactions of endosomal compartments and it may be involved in the communication between the biosynthetic and early endocytic pathways. It shares 81% identity with RAB31.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.; Authors - James W Clancy
human	WB,IHC	Clin Cancer Res; The Immune-microenvironment Confers Chemoresistance of Colorectal Cancer through Macrophage-derived IL-6.; Authors - Yuan Yin
human	WB,IF	EMBO Rep; Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomes.; Authors - Saurabh Shakya ; KD Validated
human	IF	J Cell Sci; A specific subset of RabGTPases controls cell surface exposure of MT1-MMP, extracellular matrix degradation and three-dimensional invasion of macrophages.; Authors - Wiesner Christiane C
mouse	WB	Traffic; Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes.; Authors - Seto Shintaro S
hamster, mouse, human	WB,IF	Mol Biol Cell; Rabex-5 is a Rab22 effector and mediates a Rab22-Rab5 signaling cascade in endocytosis.; Authors - Zhu Huaiping H ; KD Validated

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