RAB1B Polyclonal antibody

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  WB analysis using 17824-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17824-1-AP (RAB1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 17824-1-AP

  HAP1 (WT and RAB1A and RAB1B KO) lysates prepared with RIPA buffer, 30 μg of protein loaded. 17824-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of HeLa using 17824-1-AP

  WB result of RAB1B antibody (17824-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB1B transfected HeLa cells.

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  IHC staining of human heart using 17824-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17824-1-AP (RAB1B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 17824-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17824-1-AP (RAB1B Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 17824-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17824-1-AP (RAB1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 17824-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17824-1-AP (RAB1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 17824-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17824-1-AP (RAB1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 17824-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 17824-1-AP (RAB1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 17824-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAB1B antibody (17824-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

RAB1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17824-1-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Ras-related protein Rab-1B, Ras related protein Rab 1B, EC:3.6.5.2

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Tested Applications

• Positive WB detected in: A375 cells, HeLa cells, HAP1, A431 cells, NIH/3T3 cells
• Positive IHC detected in: human heart tissue, mouse heart tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

17824-1-AP targets RAB1B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB1B fusion protein Ag12197
• Full Name: RAB1B, member RAS oncogene family
• Calculated Molecular Weight: 201 aa, 22 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC071169
• Gene Symbol: RAB1B
• Gene ID (NCBI): 81876
• RRID: AB_2237881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H0U4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ras-related protein Rab-1B (RAB1B) belongs to the small GTPase superfamily and Rab family. Rab1b, a GTPase regulating secretory transport,  was recently associated with targeting proteins to LDs in a Drosophila RNAi screen. Rab1b recruits lipid-synthesizing enzymes, facilitating Lipid droplets (LDs) growth. The small GTPase Rab1b interacts with diacylglycerol acyltransferase 2 to facilitate its targeting to the surface of lipid droplets (PMID: 38809969). Rab1b, an extensively studied and established master regulator of ER-to-Golgi transport. The mechanism of Rab1b's function is to promote ER to LD surface targeting of those triglyceride-synthesizing enzymes  that can associate with the unique lipid monolayer comprising the LD surface (PMID: 38809969). Rab1b is a regulatory protein involved in both COPI and COPII transport (PMID: 17429068).

Publications

Species	Application	Title
human	WB	Cancer Cell; Rab1A Is an mTORC1 Activator and a Colorectal Oncogene.; Authors - Janice D Thomas
human	WB,IF	Nat Cell Biol; A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion; Authors - Yuxin Sun ; KD Validated
human	WB	Nat Cell Biol; A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.; Authors - Reiling Jan H JH
monkey	IF	Proc Natl Acad Sci U S A; Vibrio MARTX toxin processing and degradation of cellular Rab GTPases by the cytotoxic effector Makes Caterpillars Floppy; Authors - Alfa Herrera
human	IF	Dev Cell; SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport.; Authors - Rui Yan
human	IHC	Aging (Albany NY); Overexpression of Rab1B and MMP9 predicts poor survival and good response to chemotherapy in patients with colorectal cancer.; Authors - Xian-Zi Yang