RAB18 Monoclonal antibody

RAB18 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2E4B3

Cat no : 67659-1-Ig

Synonyms

RAB18, RAB18LI1, Ras related protein Rab 18

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Validation Data Gallery

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  WB analysis using 67659-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67659-1-Ig (RAB18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67659-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67659-1-Ig (RAB18 antibody) at dilution of 1:350 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67659-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67659-1-Ig (RAB18 antibody) at dilution of 1:350 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, Jurkat cells, human testis tissue, pig brain tissue, rat brain tissue, mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:175-1:700

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

67659-1-Ig targets RAB18 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB18 fusion protein Ag16669
• Full Name: RAB18, member RAS oncogene family
• Calculated Molecular Weight: 206 aa, 23 kDa
• GenBank Accession Number: BC015014
• Gene Symbol: RAB18
• Gene ID (NCBI): 22931
• RRID: AB_2882856
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	J Gastroenterol Hepatol; Effect of Rab18 on liver injury and lipid accumulation by regulating perilipin 2 and peroxisome proliferator-activated receptor gamma in non-alcoholic fatty liver disease; Authors - Lei Zhang