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Rab18 Polyclonal antibody

Rab18 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 11304-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Ras-related protein Rab-18, Ras related protein Rab 18, RAB18LI1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inmouse brain tissue, human heart tissue, human brain tissue, PC-3 cells, human testis tissue, mouse testis tissue, rat brain tissue
Positive IP detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman stomach cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11304-1-AP targets Rab18 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Rab18 fusion protein Ag1841 Predict reactive species
Full Name RAB18, member RAS oncogene family
Calculated Molecular Weight 206 aa, 23 kDa
Observed Molecular Weight 20-23 kDa
GenBank Accession NumberBC015014
Gene Symbol RAB18
Gene ID (NCBI) 22931
RRIDAB_2173932
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP72
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB18 is a member of a family of Ras-related small GTPases. RAB18 plays a role in apical endocytosis/recycling, and may be implicated in transport between the plasma membrane and early endosomes. It also plays a key role in eye and brain development and neurodegeneration. Defects in RAB18 are associated with Warburg micro syndrome type 3.

Protocols

Product Specific Protocols
WB protocol for Rab18 antibody 11304-1-APDownload protocol
IHC protocol for Rab18 antibody 11304-1-APDownload protocol
IF protocol for Rab18 antibody 11304-1-APDownload protocol
IP protocol for Rab18 antibody 11304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Clin Invest

RINT1 deficiency disrupts lipid metabolism and underlies a complex hereditary spastic paraplegia

Authors - Nathalie Launay
monkeyWB

J Cell Biol

Rab18 and a Rab18 GEF complex are required for normal ER structure.

Authors - Andreas Gerondopoulos
mouseIF

Dev Cell

KIF3B promotes a PI3K signaling gradient causing changes in a Shh protein gradient and suppressing polydactyly in mice

Authors - Shuo Wang
humanWB

PLoS Pathog

Rab18 Binds to Hepatitis C Virus NS5A and Promotes Interaction between Sites of Viral Replication and Lipid Droplets.

Authors - Salloum Shadi S
mouseWB

Food Chem

Pu-erh tea increases the metabolite Cinnabarinic acid to improve circadian rhythm disorder-induced obesity.

Authors - Shanshan Hu
humanWB,IHC

Carcinogenesis

Hepatitis B virus X protein upregulates oncogene Rab18 to result in the dysregulation of lipogenesis and proliferation of hepatoma cells.

Authors - You Xiaona X
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