Rab18 Polyclonal antibody

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  WB analysis using 11304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11304-1-AP (Rab18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 11304-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11304-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11304-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11304-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11304-1-AP

  IP result of anti-Rab18 (IP:11304-1-AP, 4ug; Detection:11304-1-AP 1:2000) with mouse testis tissue lysate 1120 ug.

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  IP experiment of rat testis using 11304-1-AP

  IP result of anti-Rab18 (IP:11304-1-AP, 4ug; Detection:11304-1-AP 1:2000) with rat testis tissue lysate 4000 ug.

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  IHC staining of mouse brain using 11304-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11304-1-AP (Rab18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11304-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11304-1-AP (Rab18 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11304-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11304-1-AP (Rab18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11304-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11304-1-AP (Rab18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 11304-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Rab18 antibody (11304-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 11304-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Rab18 antibody (11304-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 11304-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Rab18 antibody (11304-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

Rab18 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 11304-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

Ras-related protein Rab-18, Ras related protein Rab 18, RAB18LI1

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Tested Applications

• Positive WB detected in: mouse brain tissue, human heart tissue, human brain tissue, PC-3 cells, human testis tissue, mouse testis tissue, rat brain tissue
• Positive IP detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: human stomach cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 14 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Product Information

11304-1-AP targets Rab18 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Rab18 fusion protein Ag1841
• Full Name: RAB18, member RAS oncogene family
• Calculated Molecular Weight: 206 aa, 23 kDa
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC015014
• Gene Symbol: RAB18
• Gene ID (NCBI): 22931
• RRID: AB_2173932
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NP72
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB18 is a member of a family of Ras-related small GTPases. RAB18 plays a role in apical endocytosis/recycling, and may be implicated in transport between the plasma membrane and early endosomes. It also plays a key role in eye and brain development and neurodegeneration. Defects in RAB18 are associated with Warburg micro syndrome type 3.

Publications

Species	Application	Title
human	WB,IF	J Clin Invest; RINT1 deficiency disrupts lipid metabolism and underlies a complex hereditary spastic paraplegia; Authors - Nathalie Launay
monkey	WB	J Cell Biol; Rab18 and a Rab18 GEF complex are required for normal ER structure.; Authors - Andreas Gerondopoulos
mouse	IF	Dev Cell; KIF3B promotes a PI3K signaling gradient causing changes in a Shh protein gradient and suppressing polydactyly in mice; Authors - Shuo Wang
human	WB	PLoS Pathog; Rab18 Binds to Hepatitis C Virus NS5A and Promotes Interaction between Sites of Viral Replication and Lipid Droplets.; Authors - Salloum Shadi S
mouse	WB	Food Chem; Pu-erh tea increases the metabolite Cinnabarinic acid to improve circadian rhythm disorder-induced obesity.; Authors - Shanshan Hu
human	WB,IHC	Carcinogenesis; Hepatitis B virus X protein upregulates oncogene Rab18 to result in the dysregulation of lipogenesis and proliferation of hepatoma cells.; Authors - You Xiaona X ; KD Validated