RAB14 Monoclonal antibody, PBS Only

RAB14 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G7B3

Cat no : 67953-1-PBS

Synonyms

FBP, RAB 14, RAB14, Ras related protein Rab 14

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Validation Data Gallery

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  WB analysis using 67953-1-Ig (same clone as 67953-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67953-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67953-1-Ig (same clone as 67953-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67953-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67953-1-Ig (same clone as 67953-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67953-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 67953-1-Ig (same clone as 67953-1-PBS)

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67953-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67953-1-Ig (same clone as 67953-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67953-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67953-1-Ig (same clone as 67953-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67953-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67953-1-Ig (same clone as 67953-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67953-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 67953-1-Ig (same clone as 67953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67953-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67953-1-Ig (same clone as 67953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67953-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 67953-1-Ig (same clone as 67953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67953-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 67953-1-Ig (same clone as 67953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67953-1-PBS in a different storage buffer formulation.

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Product Information

67953-1-PBS targets RAB14 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB14 fusion protein Ag8167
• Full Name: RAB14, member RAS oncogene family
• Calculated Molecular Weight: 215 aa, 24 kDa
• GenBank Accession Number: BC006081
• Gene Symbol: RAB14
• Gene ID (NCBI): 51552
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.