RAB12 antibody (18843-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 18843-1-AP (same clone as 18843-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 18843-1-AP (RAB12 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 18843-1-AP (same clone as 18843-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 18843-1-AP (RAB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 18843-1-AP (same clone as 18843-1-PBS)
IP result of anti-RAB12 (IP:18843-1-AP, 4ug; Detection:18843-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 18843-1-AP (same clone as 18843-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 18843-1-AP (same clone as 18843-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 18843-1-AP (same clone as 18843-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 18843-1-AP (same clone as 18843-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 18843-1-AP (same clone as 18843-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 18843-1-AP (same clone as 18843-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 18843-1-AP (same clone as 18843-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB12 antibody (18843-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.

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Tested Applications
Product Information

Product Information

18843-1-PBS targets RAB12 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	RAB12, member RAS oncogene family
Calculated Molecular Weight	27 kDa
Observed Molecular Weight	28 kDa
GenBank Accession Number	NM_001025300
Gene Symbol	RAB12
Gene ID (NCBI)	201475
RRID	AB_10603469
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6IQ22
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.