RAB12 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

RAB12 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18843-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 18843-1-AP (same clone as 18843-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18843-1-AP (RAB12 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 18843-1-AP (same clone as 18843-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18843-1-AP (RAB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 18843-1-AP (same clone as 18843-1-PBS)

  IP result of anti-RAB12 (IP:18843-1-AP, 4ug; Detection:18843-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18843-1-AP (same clone as 18843-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18843-1-AP (same clone as 18843-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 18843-1-AP (same clone as 18843-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 18843-1-AP (same clone as 18843-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 18843-1-AP (same clone as 18843-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 18843-1-AP (same clone as 18843-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18843-1-AP (same clone as 18843-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB12 antibody (18843-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 18843-1-PBS in a different storage buffer formulation.

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Product Information

18843-1-PBS targets RAB12 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: RAB12, member RAS oncogene family
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: NM_001025300
• Gene Symbol: RAB12
• Gene ID (NCBI): 201475
• RRID: AB_10603469
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RAB12 belongs to the small GTPase superfamily and Rab family. RAB12 locates in Golgi apparatus membrane.