RAB11FIP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB11FIP2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 18136-1-AP

Synonyms

Rab11-FIP2, Rab11 FIP2, Rab11 family-interacting protein 2, NRip11

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Validation Data Gallery

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  WB analysis using 18136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18136-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18136-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 18136-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RAB11FIP2 antibody (18136-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 18136-1-AP

  Immunofluorescent analysis of HepG2 cells, using RAB11FIP2 antibody 18136-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse liver tissue, HEK-293 cells, rat brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Product Information

18136-1-AP targets RAB11FIP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB11FIP2 fusion protein Ag12736
• Full Name: RAB11 family interacting protein 2 (class I)
• Calculated Molecular Weight: 512 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC075073
• Gene Symbol: RAB11FIP2
• Gene ID (NCBI): 22841
• RRID: AB_10597110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB11FIP2, or RAB11 family interacting protein 2, is a protein that plays a crucial role in intracellular trafficking, particularly in the recycling of endocytic vesicles. It is involved in several cellular processes, including TRAM-dependent toll-like receptor 4 signaling pathway, phagocytosis, and positive regulation of GTPase activity. RAB11FIP2 is localized in endosomes, nucleoplasm, and phagocytic cups, and it is expressed ubiquitously in various tissues, with higher expression in endometrium and brain. RAB11FIP2 interacts with the actin-based motor myo5b and the small GTPase Rab11, which are key components in the regulation of vesicle tethering and transport along actin filaments. This interaction is particularly important for processes such as cell polarization, motility, and lumen formation in multicellular structures. .

Publications

Species	Application	Title
human	WB,IF	PLoS Pathog; Acquisition of Rab11 and Rab11-Fip2-A novel strategy for Chlamydia pneumoniae early survival.; Authors - Katja Mölleken ; KD Validated
human	WB	Traffic; Rab11-FIP2 Interaction with MYO5B Regulates Movement of Rab11a-Containing Recycling Vesicles.; Authors - Jenny C Schafer
human	IF	Exp Cell Res; Rab11-FIP1A regulates early trafficking into the recycling endosomes.; Authors - Jenny C Schafer