RAB10 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

21

Reviews (1)

Cat no : 11808-1-AP

Synonyms

Ras-related protein Rab-10, Ras related protein Rab 10, EC:3.6.5.2

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Validation Data Gallery

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  WB analysis using 11808-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11808-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11808-1-AP

  WB result of RAB10 antibody (11808-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB10 transfected HeLa cells.

• 

  WB analysis of NIH/3T3 using 11808-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 11808-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11808-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11808-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 11808-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11808-1-AP (RAB10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11808-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11808-1-AP (RAB10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH/3T3 using 11808-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RAB10 antibody (11808-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, NIH/3T3 cells, rat brain tissue, C6 cells, HeLa cells, MCF-7 cells, MDCK cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Product Information

11808-1-AP targets RAB10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB10 fusion protein Ag2473
• Full Name: RAB10, member RAS oncogene family
• Calculated Molecular Weight: 200 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC000896
• Gene Symbol: RAB10
• Gene ID (NCBI): 10890
• RRID: AB_2173442
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB10 belongs to the RAS superfamily of small GTPases. RAB10 localizes to a system of tubules and vesicles, and it may be involved in vesicular trafficking and neurotransmitter release. It is also associated with Glut4 translocation in adipocytes.

Publications

Species	Application	Title
rat	WB,IP,IF	Cell Res; MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development.; Authors - Xiao-Hui Xu
rat, zebrafish	WB,IF	Nat Commun; Myosin Vb controls biogenesis of post-Golgi Rab10 carriers during axon development.; Authors - Liu Yang Y
mouse	IF	Proc Natl Acad Sci U S A; Ras-related protein Rab10 facilitates TLR4 signaling by promoting replenishment of TLR4 onto the plasma membrane.; Authors - Wang Di D
mouse	WB	Curr Biol; Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axons; Authors - Sara C Sousa
rat	WB	Sci Signal; Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections.; Authors - Kong-Yan Wu
rat	WB	J Neurosci; Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.; Authors - Pavlos Nathan J NJ

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nuo (Verified Customer) (11-07-2024)

It is okay to use it to probe endogenous Rab10 but it may need a higher concentration.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse brain lysate

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