QSOX1/Quiescin Q6 Polyclonal antibody

• Featured Product
• KD/KO Validated

QSOX1/Quiescin Q6 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 12713-1-AP

Synonyms

QSOX1, UNQ2520/PRO6013, QSCN6, Q6, hQSOX

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Validation Data Gallery

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  WB analysis using 12713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12713-1-AP (QSOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12713-1-AP (QSOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 12713-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12713-1-AP (QSOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of SW 1990 using 12713-1-AP

  IP result of anti-QSOX1 (IP:12713-1-AP, 4ug; Detection:12713-1-AP 1:300) with SW 1990 cells lysate 2400ug.

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  IHC staining of human liver cancer using 12713-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12713-1-AP (QSOX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12713-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12713-1-AP (QSOX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse colon using 12713-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using QSOX1 antibody (12713-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 12713-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using QSOX1 antibody (12713-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SW 1990 cells, mouse heart tissue, human placenta tissue, rat heart tissue
• Positive IP detected in: SW 1990 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 8 publications below
• IF: See 3 publications below

Product Information

12713-1-AP targets QSOX1/Quiescin Q6 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: QSOX1/Quiescin Q6 fusion protein Ag3396
• Full Name: quiescin Q6 sulfhydryl oxidase 1
• Calculated Molecular Weight: 747 aa, 83 kDa
• Observed Molecular Weight: 66-83kDa
• GenBank Accession Number: BC017692
• Gene Symbol: QSOX1
• Gene ID (NCBI): 5768
• RRID: AB_2173314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

QSOX1(Sulfhydryl oxidase 1) catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide.It has two isoforms with the molecular weight of 67 kda and 83 kDa,respectively. It is detected a band of 95 Kda in HT1080 cell lines (PMID:17331072).Isoform 1 is expressed on golgi apparatus membrane and isoform 2 is secreted (PMID:10708601). The isoform1(QSOX1a) is the full length of QSOX1, approximately 78 kd; and the isoform2(QSOX1b) is the splicing form, approximately 62kd(PMID:14633699; 2496424). QSOX1 is highly expressed in tumor cell lines but is not expressed in adjacent normal cells (PMID:21989104).

Publications

Species	Application	Title
human	IHC	Redox Biol; Quiescin sulfhydryl oxidase 1 promotes sorafenib-induced ferroptosis in hepatocellular carcinoma by driving EGFR endosomal trafficking and inhibiting NRF2 activation.; Authors - Jialei Sun
	WB	Antioxid Redox Signal; The Emerging Role of QSOX1 in Cancer.; Authors - Douglas F Lake
human	IHC	Breast Cancer Res; High expression of QSOX1 reduces tumorogenesis, and is associated with a better outcome for breast cancer patients.; Authors - Pernodet Nicolas N
human	WB,IHC	Breast Cancer Res; Expression of quiescin sulfhydryl oxidase 1 is associated with a highly invasive phenotype and correlates with a poor prognosis in Luminal B breast cancer.; Authors - Katchman Benjamin A BA
human	IHC	Mod Pathol; QSOX1 expression is associated with aggressive tumor features and reduced survival in breast carcinomas.; Authors - Gøril Knutsvik
human	IHC	Oncotarget; Reduced QSOX1 enhances radioresistance in nasopharyngeal carcinoma.; Authors - Lei Zhou

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