QDPR Polyclonal antibody

QDPR Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 14908-1-AP

Synonyms

DHPR, Dihydropteridine reductase, HDHPR, PKU2, QDPR, SDR33C1

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Validation Data Gallery

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  WB analysis of mouse liver using 14908-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14908-1-AP (QDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14908-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14908-1-AP (QDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14908-1-AP

  IP result of anti-QDPR (IP:14908-1-AP, 4ug; Detection:14908-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of mouse brain using 14908-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14908-1-AP (QDPR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14908-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14908-1-AP (QDPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, human liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

14908-1-AP targets QDPR in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: QDPR fusion protein Ag6705
• Full Name: quinoid dihydropteridine reductase
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC000576
• Gene Symbol: QDPR
• Gene ID (NCBI): 5860
• RRID: AB_2173013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dihydropteridine reductase (QDPR), also named as DHPR and HDHPR, is an essential enzyme in the hydroxylating system of the aromatic amino acids, since it catalyses the regeneration of tetrahydrobiopterin (BH4), the natural cofactor of phenylalanine, tyrosine, and tryptophan hydroxylases, from the quininoid-dihydrobiopterin produced in these coupled reactions(PMID:8326489). The QDPR protein is active as a dimer, with a subunit Mr of 26 kDa(PMID:7627180). This protein belongs to the short-chain dehydrogenases/reductases (SDR) family. Defects in QDPR are the cause of BH4-deficient hyperphenylalaninemia type C (HPABH4C)(PMID:11153907).

Publications

Species	Application	Title
mouse	WB,IF	Glia; Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome; Authors - Sophie B Siems
mouse	WB	Mol Autism; RNA sequencing and proteomics approaches reveal novel deficits in the cortex of Mecp2-deficient mice, a model for Rett syndrome.; Authors - Natasha L Pacheco
mouse	WB	Front Mol Neurosci; Broad Influence of Mutant Ataxin-3 on the Proteome of the Adult Brain, Young Neurons, and Axons Reveals Central Molecular Processes and Biomarkers in SCA3/MJD Using Knock-In Mouse Model.; Authors - Kalina Wiatr
human	WB	Genes Genomics; Subproteomic profiling from renal cortices in OLETF rats reveals mutations of multiple novel genes in diabetic nephropathy.; Authors - Zhiguo Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (01-20-2023)

Very good antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver and mouse brain

QDPR Antibody Western Blot validation (1:1000 dilution) in Mouse liver and mouse brain (Cat no:14908-1-AP)