Enhanced Protease Inhibitor Cocktail (EDTA-Free,100X in DMSO)

Concentration:

100x

Publications

10

Cat no : PR20016

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Validation Data Gallery

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  lane1: without protease inhibitor
  lane 2-4: treated with protease inhibitor cocktail from three other companies
  lane 5: treated with Proteintech Protease Inhibitor Cocktail;
  Western Blot of U973 lysates treated with or without protease inhibitors, using antibodies of Beta actin (60008-1-Ig), TBP (66166-1-Ig), Vinculin (66305-1-Ig), GAPDH (60004-1-Ig), respectively.

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Product Information

Enhanced Protease Inhibitor Cocktail is a mix of protease inhibitors with broad specificity for various proteases, composed of Aprotinin, Bestatin, Leupetin, Pepstatin,  PMSF, E-64, Phosphoramidon to promote broad spectrum protection against serine, cysteine, and aspartic acid proteases, aminopeptidases, and metalloproteases, and can prevent protein degradation during generation of cell or tissue lysates. The protease inhibitor cocktail can be used in the applications of protein purification, immunoblot, co-immunoprecipitation, immunofluorescence, and immunohistochemistry etc.

A mix of various protease inhibitors, such as Aprotinin, Bestatin, Leupetin, Pepstatin, PMSF, E-64, Phosphoramidon etc.

Storage

Store at -20℃ for a year.

Usage

Desolved at room temperature and briefly vortex the Protease Inhibitor Cocktail (100X) before use, then dilute 1:100 (volume) in cell lysate or lysis buffer.

Cited in Article as

PR20016, Enhanced Protease Inhibitor Cocktail (EDTA-Free,100X in DMSO), Proteintech, IL, USA

Publications

Application	Title
	NPJ Biofilms Microbiomes; Impact of structurally diverse polysaccharides on colonic mucin O-glycosylation and gut microbiota; Authors - Tong Zhao
CoIP	Cell Commun Signal; Metformin alleviates bone loss in ovariectomized mice through inhibition of autophagy of osteoclast precursors mediated by E2F1; Authors - Xudong Xie
	BMC Endocr Disord; Acteoside protects podocyte against apoptosis through regulating AKT/GSK-3β signaling pathway in db/db mice; Authors - Xiaoya Li
	Toxicol Lett; Hsp47 acts as a bridge between NLRP3 inflammasome and hepatic stellate cells activation in arsenic-induced liver fibrosis; Authors - Weizhuo Yuan
	Curr Pharm Des; Mechanism of HSP90 Inhibitor in the Treatment of DSS-induced Colitis in Mice by Inhibiting MAPK Pathway and Synergistic Effect of Compound Sophora Decoction; Authors - Yuyi Yuan
	Mol Immunol; Elucidation of the underlying mechanism of Hua-ban decoction in alleviating acute lung injury by an integrative approach of network pharmacology and experimental verification; Authors - Wei Zhou