Prohibitin Monoclonal antibody, PBS Only

Prohibitin Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D3G5

Cat no : 60092-1-PBS

Synonyms

Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, PHB, PHB1, prohibitin

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Validation Data Gallery

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  WB analysis using 60092-1-Ig (same clone as 60092-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60092-1-Ig (Prohibitin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60092-1-Ig (same clone as 60092-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60092-1-Ig (PHB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 60092-1-Ig (same clone as 60092-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60092-1-Ig (PHB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60092-1-Ig (same clone as 60092-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60092-1-Ig (PHB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60092-1-Ig (Prohibitin Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60092-1-Ig (Prohibitin Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 60092-1-Ig(PHB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 60092-1-Ig(PHB antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunofluorescent analysis of Hela cells, using PHB antibody 60092-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

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Product Information

60092-1-PBS targets Prohibitin in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

• Tested Reactivity: human, pig, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Prohibitin fusion protein Ag1240
• Full Name: prohibitin
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC013401
• Gene Symbol: Prohibitin
• Gene ID (NCBI): 5245
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis (PMID: 20840588 ). This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription (PMID: 18558096). Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis (PMID: 24402549).