Prohibitin 2 Monoclonal antibody

• Featured Product
• KD/KO Validated

Prohibitin 2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

13

CloneNo.

1D9C7

Cat no : 66424-1-Ig

Synonyms

BAP, Bap37, D prohibitin, p22, PHB2, PNAS 141, prohibitin 2, REA

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Validation Data Gallery

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  WB analysis using 66424-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HeLa using 66424-1-Ig

  WB result of Prohibitin 2 antibody (66424-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Prohibitin 2 transfected HeLa cells.

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  WB analysis using 66424-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66424-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66424-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 66424-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 10x lens).

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  IHC staining of human heart using 66424-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 40x lens).

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  IF Staining of HepG2 using 66424-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). F-actin was labelled in red with Phalloidin.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, HSC-T6 cells, ROS1728 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human breast cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:10000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 7 publications below

Product Information

66424-1-Ig targets Prohibitin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Prohibitin 2 fusion protein Ag2977
• Full Name: prohibitin 2
• Calculated Molecular Weight: 299 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC014766
• Gene Symbol: Prohibitin 2
• Gene ID (NCBI): 11331
• RRID: AB_2811041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHB2, also named as BAP, REA, D-prohibitin, BAP37 and Prohibitin-2, belongs to the prohibitin family. It acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. PHB2 functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. PHB2 is probably involved in regulating mitochondrial respiration activity and in aging.(PMID: 10359819)

Publications

Species	Application	Title
human	WB	EMBO Rep; NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.; Authors - Norihiko Furuya
human	WB	Elife; Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.; Authors - Maxence Le Vasseur
mouse	WB,IF	Environ Pollut; Fluoride induced mitochondrial impairment and PINK1-mediated mitophagy in Leydig cells of mice: In vivo and in vitro studies.; Authors - Chen Liang
human	WB,IF	Viruses; Interaction between PHB2 and Enterovirus A71 VP1 Induces Autophagy and Affects EV-A71 Infection.; Authors - Weitao Su ; KD Validated
human	IHC	Mol Biol Cell; Discovery of bactericides as an acute mitochondrial membrane damage inducer.; Authors - Ryan Houston
human	IF	PLoS One; A549 cells contain enlarged mitochondria with independently functional clustered mtDNA nucleoids.; Authors - Aleksandrs Nasonovs