Progranulin/PGRN Polyclonal antibody, PBS Only

Progranulin/PGRN Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10053-1-PBS

Synonyms

granulin, Granulin-1, Granulin precursor, Glycoprotein of 88 Kda, Glycoprotein 88

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Validation Data Gallery

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  WB analysis using 10053-1-AP (same clone as 10053-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10053-1-AP (Granulin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10053-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10053-1-AP (same clone as 10053-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10053-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10053-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 10053-1-AP (same clone as 10053-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10053-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10053-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10053-1-AP (same clone as 10053-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10053-1-AP (Granulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10053-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10053-1-AP (same clone as 10053-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10053-1-AP (Granulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10053-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 10053-1-AP (same clone as 10053-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10053-1-AP (Granulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10053-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 10053-1-AP (same clone as 10053-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10053-1-AP (Granulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10053-1-PBS in a different storage buffer formulation.

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Product Information

10053-1-PBS targets Progranulin/PGRN in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Progranulin/PGRN fusion protein Ag0010
• Full Name: granulin
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC010577
• Gene Symbol: Granulin
• Gene ID (NCBI): 2896
• RRID: AB_514063
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system. Several in vitro studies have revealed that PGRN is classified into two isoforms according to its glycosylation status, the glycosylated immature isoform (58-68 kDa) and the fully glycosylated mature secretory isoform (∼88 kDa) (PMID: 25838514).