Prealbumin/transthyretin Monoclonal antibody, PBS Only

Prealbumin/transthyretin Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG2a

Reactivity

mouse, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1H8C4

Cat no : 66108-1-PBS

Synonyms

Prealbumin, transthyretin, TTR, TBPA, PALB

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Validation Data Gallery

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  WB analysis of human brain using 66108-1-Ig (same clone as 66108-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66108-1-Ig (Prealbumin/transthyretin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 66108-1-Ig (same clone as 66108-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 66108-1-Ig (same clone as 66108-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66108-1-Ig (same clone as 66108-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66108-1-Ig (same clone as 66108-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66108-1-Ig (same clone as 66108-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66108-1-Ig (same clone as 66108-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-12 using 66108-1-Ig (same clone as 66108-1-PBS)

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Prealbumin/transthyretin (66108-1-Ig, Clone:1H8C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

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Product Information

66108-1-PBS targets Prealbumin/transthyretin in Indirect ELISA, IHC, WB applications and shows reactivity with mouse, human samples.

• Tested Reactivity: mouse, human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Prealbumin/transthyretin fusion protein Ag19232
• Full Name: transthyretin
• Calculated Molecular Weight: 147 aa, 16 kDa
• Observed Molecular Weight: 14-16 kDa, 28-33 kDa
• GenBank Accession Number: BC020791
• Gene Symbol: TTR
• Gene ID (NCBI): 7276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Transthyretin (TTR) is a plasma transport protein for thyroxine and retinol, through the association with retinol-binding protein. It is a homotetrameric protein synthesized mainly in liver, choroid plexus, retinal pigment epithelium, and pancreas.Within the CNS, TTR is the only known protein synthesized solely by the choroid plexus. Mutant and wildtype TTR give rise to various forms of amyloid deposition (amyloidosis). Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR),hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) and carpal tunnel syndrome type 1 (CTS1). In addition, positive immunostaining for TTR has been reported as a sensitive diagnostic marker of choroid plexus tumors. TTR can be expressed as monomer (14-16 kDa), dimmer (28-33 kDa), or tetramer around 55-60 kDa. (PMID: 22103483)