Plasminogen Polyclonal antibody, PBS Only

Plasminogen Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17462-1-PBS

Synonyms

PLG, Plasmin heavy chain A, short form, EC:3.4.21.7, Angiostatin

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Validation Data Gallery

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  WB analysis of human blood using 17462-1-AP (same clone as 17462-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 17462-1-AP (Plasminogen antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17462-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 17462-1-AP (same clone as 17462-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17462-1-AP (Plasminogen antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17462-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 17462-1-AP (same clone as 17462-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17462-1-AP (Plasminogen antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17462-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 17462-1-AP (same clone as 17462-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17462-1-AP (Plasminogen antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17462-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 17462-1-AP (same clone as 17462-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 17462-1-AP (Plasminogen antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17462-1-PBS in a different storage buffer formulation.

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Product Information

17462-1-PBS targets Plasminogen in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Plasminogen fusion protein Ag11217
• Full Name: plasminogen
• Calculated Molecular Weight: 810 aa, 91 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC060513
• Gene Symbol: Plasminogen
• Gene ID (NCBI): 5340
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.