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Plasminogen Polyclonal antibody

Plasminogen Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17462-1-AP

Synonyms

PLG, Plasmin heavy chain A, short form, EC:3.4.21.7, Angiostatin

Validation Data Gallery

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  • Western Blot (WB) analysis of human blood using Plasminogen Polyclonal antibody (17462-1-AP)

    WB analysis of human blood using 17462-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 17462-1-AP (Plasminogen antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Plasminogen Polyclonal antibody (17462-1-AP)

    WB analysis of human placenta using 17462-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 17462-1-AP (Plasminogen antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Plasminogen Polyclonal antibody (17462-1-AP)

    WB analysis of human placenta using 17462-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 17462-1-AP (Plasminogen antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Plasminogen Polyclonal antibody (17462-1-AP)

    IHC staining of human stomach using 17462-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17462-1-AP (Plasminogen antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Plasminogen Polyclonal antibody (17462-1-AP)

    IF Staining of HepG2 using 17462-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 17462-1-AP (Plasminogen antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman blood tissue, human placenta tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

17462-1-AP targets Plasminogen in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Plasminogen fusion protein Ag11217
Full Name plasminogen
Calculated Molecular Weight 810 aa, 91 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC060513
Gene Symbol Plasminogen
Gene ID (NCBI) 5340
RRIDAB_10644136
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.

Protocols

Product Specific Protocols
WB protocol for Plasminogen antibody 17462-1-APDownload protocol
IHC protocol for Plasminogen antibody 17462-1-APDownload protocol
IF protocol for Plasminogen antibody 17462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Front Microbiol

Binding of Plasminogen to Streptococcus suis Protein Endopeptidase O Facilitates Evasion of Innate Immunity in Streptococcus suis.

Authors - Yang Zhou
View Article
mouseWB

Blood

A mechanism for hereditary angioedema with normal C1 inhibitor: an inhibitory regulatory role for the factor XII heavy chain

Authors - Ivan Ivanov
View Article
mouseWB

Nat Commun

Fibrinolytic-deficiencies predispose hosts to septicemia from a catheter-associated UTI

Authors - Jonathan J Molina
View Article