Plakophilin 2 Polyclonal antibody

Plakophilin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26479-1-AP

Synonyms

PKP2, Plakophilin-2

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Validation Data Gallery

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  WB analysis using 26479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26479-1-AP (Plakophilin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 26479-1-AP

  IP result of anti-Plakophilin 2 (IP:26479-1-AP, 4ug; Detection:26479-1-AP 1:1000) with HepG2 cells lysate 2200 ug.

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  IHC staining of human ovary cancer using 26479-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26479-1-AP (Plakophilin 2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Caco-2 using 26479-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Plakophilin 2 antibody (26479-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: Caco-2 cells, HepG2 cells, rat heart tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

26479-1-AP targets Plakophilin 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Plakophilin 2 fusion protein Ag24155
• Full Name: plakophilin 2
• Calculated Molecular Weight: 837 aa, 93 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC094762
• Gene Symbol: Plakophilin 2
• Gene ID (NCBI): 5318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plakophilin 2 also known as PKP2, belongs to the beta-catenin family. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton (PMID: 10828611). Mutations in PKP2 are associated with Arrhythmogenic right ventricular dysplasia, familial, 9 (ARVD9)(PMID: 15489853).

Publications

Species	Application	Title
mouse	WB,IF	J Nanobiotechnology; ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via mTORC1-TFEB-BLOC1S3 axis; Authors - Xuan Lai
human	WB	J Neurooncol; The effects of BMP2 and the mechanisms involved in the invasion and angiogenesis of IDH1 mutant glioma cells; Authors - Hui Xu