Various lysates were subjected to SDS PAGE followed by western blot with 26479-1-AP (Plakophilin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 26479-1-AP (Plakophilin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 26479-1-AP
IP result of anti-Plakophilin 2 (IP:26479-1-AP, 4ug; Detection:26479-1-AP 1:1000) with HepG2 cells lysate 2200 ug.
IP result of anti-Plakophilin 2 (IP:26479-1-AP, 4ug; Detection:26479-1-AP 1:1000) with HepG2 cells lysate 2200 ug.
IHC staining of human ovary cancer using 26479-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26479-1-AP (Plakophilin 2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26479-1-AP (Plakophilin 2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Caco-2 using 26479-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Plakophilin 2 antibody (26479-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Plakophilin 2 antibody (26479-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
Caco-2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:6000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Plakophilin 2 also known as PKP2, belongs to the beta-catenin family. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton (PMID: 10828611). Mutations in PKP2 are associated with Arrhythmogenic right ventricular dysplasia, familial, 9 (ARVD9)(PMID: 15489853).
Various lysates were subjected to SDS PAGE followed by western blot with 26479-1-AP (Plakophilin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human ovary cancer using 26479-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26479-1-AP (Plakophilin 2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HepG2 using 26479-1-AP
IP result of anti-Plakophilin 2 (IP:26479-1-AP, 4ug; Detection:26479-1-AP 1:1000) with HepG2 cells lysate 2200 ug.
IF/ICC Figures
IF Staining of Caco-2 using 26479-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Plakophilin 2 antibody (26479-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Plakophilin 2 Polyclonal antibody
Catalog Number
26479-1-AP
Citations
2
Dilutions
WB : 1:1000-1:6000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, rat, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
