Placental lactogen Monoclonal antibody

Placental lactogen Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D11E4

Cat no : 66728-1-Ig

Synonyms

Choriomammotropin, CS 2, CSB, CSH1, CSH2, hCS B, Lactogen, PL, Placental lactogen

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Validation Data Gallery

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  WB analysis of human placenta using 66728-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66728-1-Ig (Placental lactogen antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 66728-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66728-1-Ig (Placental lactogen antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66728-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66728-1-Ig (Placental lactogen antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66728-1-Ig targets Placental lactogen in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: Placental lactogen fusion protein Ag9412
• Full Name: chorionic somatomammotropin hormone 2
• Calculated Molecular Weight: 217 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC022044
• Gene Symbol: CSH2
• Gene ID (NCBI): 1443
• RRID: AB_2882078
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human growth hormone (GH) locus is comprised by two GH (GH1 and GH2) genes and three chorionic somatomammotropin (CSH1, CSH2 and CSH-L) genes. Placental lactogens (CSH1, CSH2) are produced in the mammalian placenta and are similar in structure and function to growth hormones. Together, placental lactogens and growth factors play an essential role to assure successful lactation after pregnancy. Placental lactogens also modify the metabolic state of the mother during pregnancy to supply energy to the fetus. Both CSH1 and CSH2 can be detected by this antibody.