Phospholipase C epsilon 1 Polyclonal antibody

Phospholipase C epsilon 1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 55463-1-AP

Synonyms

KIAA1516, Phospholipase C epsilon 1, phospholipase C, epsilon 1, PLC epsilon 1, PLCE, PLCE1, PPLC

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Validation Data Gallery

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  IHC staining of human kidney using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human oesophagus cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human kidney tissue, human oesophagus cancer tissue, human stomach cancer tissue, human colon tissue, human colon cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55463-1-AP targets Phospholipase C epsilon 1 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phospholipase C, epsilon 1
• Calculated Molecular Weight: 257 kDa
• GenBank Accession Number: NM_016341
• Gene Symbol: PLCE1
• Gene ID (NCBI): 51196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospholipase C epsilon 1 (PLCE1) is also named as KIAA1516, PLCE and PPLC. TRAPPC10 (Cyclin-J) Belongs to the Cyclin family. PLCE1, which mediates small GTPases of the Ras superfamily through the activity of its Ras guanine exchange factor, is a bifunctional enzyme (PMID: 31867717). As the effector of heterotrimer and small G protein, it is involved in regulating cell growth, cell survival, T-cell activation, and actin organization (PMID: 32238860). PLCE1 is identified as a member of phospholipase family, and PLCE1 was upregulated in LPS-induced pneumonia cells (PMID: 35527658). PLCE1 is involved in the regulation of Rho GTPases and MAPKs in podocytes, and PLCE1 interacts with NCK2 but not with NCK1 (PMID: 32238860). PLCE1 is a multifunctional enzyme exhibiting phospholipase as well as RasGEF activities (PMID: 11022047).