Phospho-p70(S6K) (Thr229) Recombinant antibody, PBS Only

Phospho-p70(S6K) (Thr229) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G19

Cat no : 81592-1-PBS

Synonyms

p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K), p70(S6K) alpha, P70S6K1, PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A

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Validation Data Gallery

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  WB analysis of MCF-7 using 81592-1-RR (same clone as 81592-1-PBS)

  Non-treated and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 81592-1-RR (Phosoho-p70(S6K) (Thr229) antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with 60004-1-Ig (GAPDH antibody) as loading control. This data was developed using the same antibody clone with 81592-1-PBS in a different storage buffer formulation.

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Product Information

81592-1-PBS targets Phospho-p70(S6K) (Thr229) in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: ribosomal protein S6 kinase, 70kDa, polypeptide 1
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 65-85 kDa
• GenBank Accession Number: BC053365
• Gene Symbol: p70 S6K
• Gene ID (NCBI): 6198
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA, and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of mTOR and acts as a crucial effector of mTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K). The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation, and metabolism and is the central regulator of translation of some components of the protein synthesis system. Due to alternative translation, two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1).