Phospho-mTOR (Ser2481) Polyclonal antibody

Phospho-mTOR (Ser2481) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 28879-1-AP

Synonyms

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, Phospho-mTOR (Ser2481), RAFT1, Rapamycin target protein 1, RAPT1

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Validation Data Gallery

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  WB analysis using 28879-1-AP

  Nontreated and insulin-treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28879-1-AP (Phospho-mTOR (Ser2481) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Insulin treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

28879-1-AP targets Phospho-mTOR (Ser2481) in WB, ELISA applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: FK506 binding protein 12-rapamycin associated protein 1
• Calculated Molecular Weight: 289 kDa
• Observed Molecular Weight: 250-289 kDa
• GenBank Accession Number: BC117166
• Gene Symbol: mTOR
• Gene ID (NCBI): 2475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTOR, also named FRAP1, FRAP, FRAP2, and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is the kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. mTORC2 is also activated by growth factors but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.

Publications

Species	Application	Title
mouse	WB	J Ethnopharmacol; Iridoids from Gardeniae fructus ameliorates depression by enhancing synaptic plasticity via AMPA receptor-mTOR signaling; Authors - Baomei Xia
mouse	WB	Chem Biol Interact; Oral administration of sophoricoside (SOP) inhibits neuronal damage and neuroinflammation to curb neurodegeneration in Parkinson's disease; Authors - Hefei Wang
mouse	WB	Biochem Pharmacol; Targeting FTO suppresses hepatocellular carcinoma by inhibiting ERBB3 and TUBB4A expression; Authors - Lingli Jiang
mouse	WB	Front Pharmacol; Revealing the role of metformin in gastric intestinal metaplasia treatment; Authors - Ruoyu Hu
human	WB	Cell Rep; Synergistic hyperactivation of both mTORC1 and mTORC2 underlies the neural abnormalities of PTEN-deficient human neurons and cortical organoids; Authors - Navroop K Dhaliwal