Phospho-eEF2K (Thr57) Polyclonal antibody

Phospho-eEF2K (Thr57) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30811-1-AP

Synonyms

eEF 2 kinase, eEF 2K, EEF2K, HSU93850, Phospho-eEF2K (Thr57)

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Validation Data Gallery

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  WB analysis using 30811-1-AP

  Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 30811-1-AP (Phospho-eEF2K (Thr57) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

• 

  WB analysis using 30811-1-AP

  Non-treated NIH/3T3 cells and Forskolin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 30811-1-AP (Phospho-eEF2K (Thr57) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, λ phosphatase treated HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30811-1-AP targets Phospho-eEF2K (Thr57) in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: eukaryotic elongation factor-2 kinase
• Calculated Molecular Weight: 725 aa, 100 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC032665
• Gene Symbol: EEF2K
• Gene ID (NCBI): 29904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic elongation factor‐2 kinase (eEF2K), well‐known to be a Ca2+ calmodulin (CaM)‐dependent kinase, is overexpressed in several types of malignancies, most notably Triple negative breast cancer (TNBC). Increasing evidence indicates that eEF2K may modulate the expression of some apoptotic proteins such as XIAP, c‐FLIPL, Bcl‐XL, PI3KCI and p70S6K to inhibit apoptotic process in cancer. On the other hand, it may regulate autophagy involved in AMPK, mTORC1 and ULK1, thereby promoting cancer cell survival. (PMID: 29094413)