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Phospho-c-MYC (Ser62) Polyclonal antibody, PBS Only

Phospho-c-MYC (Ser62) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28915-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-c-MYC (Ser62) Polyclonal antibody (28915-1-AP)

    WB analysis using 28915-1-AP (same clone as 28915-1-PBS)

    Non-treated and MG132 treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28915-1-AP (Phospho-c-MYC (Ser62) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28915-1-PBS in a different storage buffer formulation.

This site is also equivalent to Ser77 for c-MYC isoform 2 (P01106-2).

Product Information

28915-1-PBS targets Phospho-c-MYC (Ser62) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated Molecular Weight 49 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC000141
Gene Symbol MYC
Gene ID (NCBI) 4609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MYC is a transcription factor that globally enhances expression of transcribing genes, including those vital for cell cycle, growth, proliferation, and survival in normal and cancer cells. MYC has multiple isomers, and this antibody recognizes the phosphorylation of the 439 amino acid isomer (P01106-1, UniProt) at the 62 serine site. Posttranslational modifications that regulate MYC stability include phosphorylations at Ser62 and Thr58. Phosphorylation at Ser62, primarily by ERK, stabilizes MYC. While kinases other than ERK phosphorylate Ser62, GSK3β has been the only kinase known to phosphorylate MYC at Thr58.(PMID: 32482868)