Phospho-VPS34 (Thr159) Polyclonal antibody

• 

  WB analysis using 29675-1-AP

  Non-treated SH-SY5Y cells, phosphatase inhibitor treated SH-SY5Y cells cells and λ phosphatase treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 29675-1-AP (Phospho-VPS34 (Thr159) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-VPS34 (Thr159) Polyclonal Antibody for WB, ELISA

Cat No. 29675-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, λ phosphatase treated SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29675-1-AP targets Phospho-VPS34 (Thr159) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phosphoinositide-3-kinase, class 3
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: NM_002647
• Gene Symbol: VPS34
• Gene ID (NCBI): 5289
• RRID: AB_3669693
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NEB9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS34 phosphorylates phosphatidylinositol to produce PtdIns3P and is the progenitor of the phosphoinositide 3-kinase (PI3K) family. VPS34 has a simpler domain organization than class I PI3Ks, which belies the complexity of its quaternary organization, with the enzyme always functioning within larger assemblies. (PMID: 30397185)

Publications

Species	Application	Title
human	WB	Eur J Med Chem; Discovery of a potent anticancer agent against pancreatic ductal adenocarcinoma targeting FAK with DFG-out state and JAK/Aurora kinases; Authors - Rong-Hong Zhang