Phospho-ULK1 (Thr468) Polyclonal antibody

Phospho-ULK1 (Thr468) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 29006-1-AP

Synonyms

ATG1, FLJ38455, FLJ46475, KIAA0722, ULK1, Unc 51 like kinase 1, UNC51, Unc51.1

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Validation Data Gallery

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  WB analysis using 29006-1-AP

  Non-treated HeLa, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 29006-1-AP (Phospho-ULK1 (Thr468) antibody) at dilution of 1:4000 incubated at room temperature for 1 hours.

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Tested Applications

• Positive WB detected in: λ phosphatase treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

29006-1-AP targets Phospho-ULK1 (Thr468) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: unc-51-like kinase 1 (C. elegans)
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: NM_003565
• Gene Symbol: ULK1
• Gene ID (NCBI): 8408
• RRID: AB_2918229
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. S467, S556, and T574 are sites responsible for regulating mitochondrial homeostasis during starvation. (PMID: 30517873, PMID: 21258367)

Publications

Species	Application	Title
rat	WB	Brain Res Bull; N-linoleyltyrosine protects neurons against Aβ1-40-induced cell toxicity via autophagy involving the CB2/AMPK/mTOR/ULK1 pathway; Authors - Yi Zhou
human	WB	Environ Toxicol; Lysine specific demethylase 1 inhibits sodium arsenite activation of HSCs by regulating SESN2/AMPK/ULK1 signaling pathway activity; Authors - Yingwan Zhang