Phospho-TBC1D1 (Ser660) Polyclonal antibody

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  WB analysis using 29034-1-AP

  Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 29034-1-AP (Phospho-TBC1D1 (Ser660) antibody) at dilution of 1:10000 incubated at room temperature for 1 hours.

Phospho-TBC1D1 (Ser660) Polyclonal Antibody for WB, ELISA

Cat No. 29034-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

KIAA1108, Phospho-TBC1D1 (Ser660), TBC, TBC1, TBC1 domain family member 1, TBC1D1

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Tested Applications

• Positive WB detected in: Calyculin A treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29034-1-AP targets Phospho-TBC1D1 (Ser660) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
• Calculated Molecular Weight: 133 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC050321
• Gene Symbol: TBC1D1
• Gene ID (NCBI): 23216
• RRID: AB_2918232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86TI0
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA , pH 7.3
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Background Information

TBC1D1 belong to the family of RabGAPs and its physiological roles in glucose homeostasis and its implication in human diseases have already been characterized in skeletal muscle and adipocytes in a large number of independent studies. TBC1D1 including twophospho-tyrosine-binding (PTB) domains, a calmodulin-binding domain(CBD) and the func-tional GAP domain responsible for catalyzing GTP hydrolysis. TBC1D1 is phosphorylated by AMPK at Ser660 and Thr237, and by AKT at Thr596. The RabGAPs TBC1D1 is important regulators of glucose homeostasis inskeletal muscle and adipose tissue. (PMID: 31627187, PMID: 18477703)