Phospho-STK11/LKB1 (Thr189) Polyclonal antibody

Phospho-STK11/LKB1 (Thr189) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 28965-1-AP

Synonyms

LKB1, Phospho-STK11 (Thr189), PJS, serine/threonine kinase 11, STK11

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Validation Data Gallery

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  WB analysis using 28965-1-AP

  Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28965-1-AP (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:10000 incubated at room temperature for 1 hours.

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  WB analysis using 28965-1-AP

  Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28965-1-AP (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

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Tested Applications

• Positive WB detected in: Calyculin A treated PC-3 cells, UV treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

28965-1-AP targets Phospho-STK11/LKB1 (Thr189) in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: serine/threonine kinase 11
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC007981
• Gene Symbol: LKB1
• Gene ID (NCBI): 6794
• RRID: AB_2918225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Liver kinase 1 (LKB1 also known as STK11) is a tumor suppressor gene that is inactivated by bi-allelic mutation in non-small cell lung cancer (NSCLC), malignant melanoma and cervical cancer. LKB1 protein has a central kinase domain, two N-terminal nuclear leading sequences, and a C-terminal regulatory motif. The protein also contains a CAAX motif at C-terminal end and can be farnesylated. The formation of an LKB1/Strad/MO25 complex is essential for LKB1's kinase activity, and there are at least 13 known substrates of LKB1, which includes AMPKs, BRSKs, and MARKs. LKB1 is the major kinase that phosphorylates the AMPK activation loop in conditions of energy stress. (PMID: 25679014, PMID: 19629071)

Publications

Species	Application	Title
human	WB	Cells; Psoralen Suppresses Lipid Deposition by Alleviating Insulin Resistance and Promoting Autophagy in Oleate-Induced L02 Cells.; Authors - Yuhao Wang