Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Monoclonal antibody, PBS Only

Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F7G5

Cat no : 68000-1-PBS

Synonyms

STAT5A, STAT5, MGF, 1F7G5

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Validation Data Gallery

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  WB analysis using 68000-1-Ig (same clone as 68000-1-PBS)

  Non-treated and GM-CSF treated TF-1 cells were subjected to SDS PAGE followed by western blot with 68000-1-lg (Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control. This data was developed using the same antibody clone with 68000-1-PBS in a different storage buffer formulation.

• 

  FC experiment of TF-1 using 68000-1-Ig (same clone as 68000-1-PBS)

  1x10^6 GM-CSF treated TF-1 cells were intracellularly stained with 0.13 ug Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Monoclonal antibody (68000-1-Ig, Clone:1F7G5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.13 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 68000-1-PBS in a different storage buffer formulation.

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Product Information

68000-1-PBS targets Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: signal transducer and activator of transcription 5A
• Calculated Molecular Weight: 794 aa, 92 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC027036
• Gene Symbol: STAT5A
• Gene ID (NCBI): 6776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human STAT5A.