Phospho-STAT3 (Ser727) Monoclonal antibody, PBS Only

Phospho-STAT3 (Ser727) Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G11G11

Cat no : 60479-1-PBS

Synonyms

STAT3, STAT 3, HIES, APRF, Acute-phase response factor

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Validation Data Gallery

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  WB analysis using 60479-1-Ig (same clone as 60479-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60479-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60479-1-Ig (same clone as 60479-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60479-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60479-1-Ig (same clone as 60479-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60479-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60479-1-Ig (same clone as 60479-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60479-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 60479-1-Ig (same clone as 60479-1-PBS)

  UV treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (STAT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60479-1-PBS in a different storage buffer formulation.

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Product Information

60479-1-PBS targets Phospho-STAT3 (Ser727) in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: signal transducer and activator of transcription 3 (acute-phase response factor)
• Calculated Molecular Weight: 770 aa, 88 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: BC000627
• Gene Symbol: STAT3
• Gene ID (NCBI): 6774
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis.