Phospho-STAT1 (Ser727) Recombinant antibody

Phospho-STAT1 (Ser727) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240897D10

Cat no : 80683-7-RR

Synonyms

phospho-S727 STAT1, Phospho STAT1 (Ser727), Phospho STAT1, p Stat1 (S727), phospho-STAT1

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Validation Data Gallery

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  WB analysis using 80683-7-RR

  Non treated and IFN gamma treated HeLa cells were subjected to SDS PAGE followed by western blot with 80683-7-RR (Phospho-STAT1 (Ser727) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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Tested Applications

• Positive WB detected in: IFN gamma treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80683-7-RR targets Phospho-STAT1 (Ser727) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: signal transducer and activator of transcription 1, 91kDa
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 84-91 kDa
• GenBank Accession Number: BC002704
• Gene Symbol: STAT1
• Gene ID (NCBI): 6772
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 activating phosphorylation at Ser727 is p38-dependant duringlipotoxic stress. MLK3 activation triggers a MAPK signaling cascade resulting in STAT1 Ser727 phosphorylation. The phosphorylation of STAT1 Ser727 increases its nuclear localization, and transcriptional activity. (PMID: 28262979).