Phospho-STAT1 (Ser727) Polyclonal antibody
Phospho-STAT1 (Ser727) Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (2)
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 28977-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | IFN gamma treated HeLa cells |
| Positive IF/ICC detected in | IFN alpha treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
Product Information
28977-1-AP targets Phospho-STAT1 (Ser727) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | signal transducer and activator of transcription 1, 91kDa |
| Calculated Molecular Weight | 83 kDa |
| Observed Molecular Weight | 84 kDa |
| GenBank Accession Number | BC002704 |
| Gene Symbol | STAT1 |
| Gene ID (NCBI) | 6772 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 activating phosphorylation at Ser727 is p38-dependant during lipotoxic stress. MLK3 activation triggers a MAPK signaling cascade resulting in STAT1 Ser727 phosphorylation. The phosphorylation of STAT1 Ser727 increases its nuclear localization, and transcriptional activity. (PMID: 28262979).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-STAT1 (Ser727) antibody 28977-1-AP | Download protocol |
| IF protocol for Phospho-STAT1 (Ser727) antibody 28977-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gut Microbes Synergistic activity of Enterococcus Faecium-induced ferroptosis via expansion of IFN-γ+CD8+ T cell population in advanced hepatocellular carcinoma treated with sorafenib | ||
Heliyon SMG9 is a novel prognostic-related biomarker in glioma correlating with ferroptosis and immune infiltrates | ||
J Asthma Sodium houttuyfonate Modulates the Lung Th1/Th2 Balance and Gut Microbiota to Protect Against Pathological Changes in Lung of Ovalbumin-induced Asthmatic mice | ||
J Virol Classical swine fever virus non-structural protein 4A recruits dihydroorotate dehydrogenase to facilitate viral replication | ||
Res Sq The RhoA GTPase regulates Type I Interferon Signaling in Systemic lupus erythematosus | ||
Cancer Res Commun Differential Regulation of the Stimulator of Interferon Genes pathway in Human Papillomavirus positive and negative Head and Neck Cancers |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Emma (Verified Customer) (11-24-2022) | Works well for western blots. Only had a few unspecific bands but the antibody showed nice results for a phosphorylated protein.
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