Phospho-SMAD9 (Ser465/467) Recombinant antibody

Phospho-SMAD9 (Ser465/467) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240470A12

Cat no : 80144-2-RR

Synonyms

p SMAD9, Pho SMAD9, Pho-SMAD9, Phospho SMAD9 (Ser465/467), Phospho SMAD9 Ser465/467

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Validation Data Gallery

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  WB analysis of HeLa using 80144-2-RR

  BMP2 treated HeLa cells were subjected to SDS PAGE followed by western blot with 80144-2-RR (SMAD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: BMP2 treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80144-2-RR targets Phospho-SMAD9 (Ser465/467) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: SMAD family member 9
• Calculated Molecular Weight: 430 aa, 49 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC011559
• Gene Symbol: SMAD9
• Gene ID (NCBI): 4093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mothers against decapentaplegic homolog 9 also known as SMAD9, SMAD8, and MADH6, belongs to the SMAD family of proteins, which belong to the TGFβ superfamily of modulators. Like many other TGFβ family members, SMAD9 is involved in cell signalling. When a bone morphogenetic protein binds to a receptor (BMP type 1 receptor kinase) it causes SMAD9 to interact with SMAD anchor for receptor activation (SARA).The binding of ligands causes the phosphorylation of the SMAD9 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD9 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase. There are two isoforms of the protein.