Phospho-SMAD1 (Ser195) Recombinant antibody, PBS Only

Phospho-SMAD1 (Ser195) Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1K6

Cat no : 80257-1-PBS

Synonyms

BSP 1, BSP1, hSMAD1, JV4 1, JV41, MAD homolog 1, Mad related protein 1, MADH1, MADR1, Mothers against DPP homolog 1, SMAD 1, SMAD family member 1, SMAD1

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Validation Data Gallery

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  WB analysis using 80257-1-RR (same clone as 80257-1-PBS)

  Non-treated and UV treated A431 cells were subjected to SDS PAGE followed by western blot with 80257-1-RR (Phospho-SMAD1 (Ser195) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80257-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80257-1-RR (same clone as 80257-1-PBS)

  Non-treated and BMP2 (HZ-1128) treated HepG2 cells were subjected to SDS PAGE followed by western blot with 80257-1-RR (Phospho-SMAD1 (Ser195) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80257-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80257-1-PBS targets Phospho-SMAD1 (Ser195) in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: SMAD family member 1
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC001878
• Gene Symbol: SMAD1
• Gene ID (NCBI): 4086
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SMAD family member 1 (Smad1) have been involved in metastatic progression of many cancer types. Smad1 can be induced by many tumor-stimulating cytokines such as the bone morphogenetic protein 2 (BMP2) and TNFα and plays important roles in cell invasion and metastasis. BMP2 signalling is initiated by binding to its specific receptors, which leads to in the phosphorylation and nuclear translocation of Smad1. Translocated Smad1 then modulates the expression of downstream osteogenic genes. (PMID: 32954678, PMID: 30008908)