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Phospho-SMAD1 (Ser187) Polyclonal antibody, PBS Only

Phospho-SMAD1 (Ser187) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28865-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-SMAD1 (Ser187) Polyclonal antibody (28865-1-AP)

    WB analysis using 28865-1-AP (same clone as 28865-1-PBS)

    Non-treated HepG2 and BMP2 (HZ-1128) treated HepG2 cells were subjected to SDS PAGE followed by western blot with 28865-1-AP (Phospho-SMAD1 (Ser187) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28865-1-PBS in a different storage buffer formulation.

Product Information

28865-1-PBS targets Phospho-SMAD1 (Ser187) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name SMAD family member 1
Calculated Molecular Weight 52 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC001878
Gene Symbol SMAD1
Gene ID (NCBI) 4086
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS).