Phospho-SAMHD1 (Thr592) Polyclonal antibody

Phospho-SAMHD1 (Thr592) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31597-1-AP

Synonyms

phospho-SAMHD1, Phospho-SAMHD1-Thr592, phospho-T592-SAMHD1, p-SAMHD1, p-SAMHD1 (Thr592)

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Validation Data Gallery

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  WB analysis using 31597-1-AP

  Non-treated THP-1 cells, phosphatase inhibitor treated THP-1 cells and λ phosphatase treated THP-1 cells were subjected to SDS PAGE followed by western blot with 31597-1-AP (Phospho-SAMHD1 (Thr592) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with SAMHD1 (12586-1-AP) antibody as a loading control.

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Tested Applications

• Positive WB detected in: THP-1 cells, λ phosphatase treated THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31597-1-AP targets Phospho-SAMHD1 (Thr592) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SAM domain and HD domain 1
• Calculated Molecular Weight: 626 aa, 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC036450
• Gene Symbol: SAMHD1
• Gene ID (NCBI): 25939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAMHD1 is a GTP/dGTP-activated deoxynucleotide triphosphohydrolase. Human SAMHD1 is a 626 amino acid protein comprising an N-terminal nuclear localisation signal, two major structural domains. SAMHD1 has been reported to have alternative nuclease and/or RNA binding activities that, dependent on the cellular circumstances, can also mediate restriction of HIV-1. In addition, restriction is regulated by Threonine 592 phosphorylation and removing this regulation may enable SAMHD1 to inhibit HIV-1 in cycling cells.