Phospho-RPS6KA1 (Thr359/Ser363) Monoclonal antibody, PBS Only

Phospho-RPS6KA1 (Thr359/Ser363) Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C11D9

Cat no : 68041-1-PBS

Synonyms

HU 1, MAPKAP kinase 1a, MAPKAPK 1a, MAPKAPK1A, p90 RSK 1, p90RSK1, p90S6K, Phospho-RPS6KA1 (Thr359/Ser363), Ribosomal S6 kinase 1, RPS6KA1, RSK, RSK 1, RSK1, S6K alpha 1

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Validation Data Gallery

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  WB analysis using 68041-1-Ig (same clone as 68041-1-PBS)

  Non-treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68041-1-Ig (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta-Tubulin (HRP-66240) as loading control. This data was developed using the same antibody clone with 68041-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68041-1-Ig (same clone as 68041-1-PBS)

  Non-treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 68041-1-Ig (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta-Tubulin (HRP-66240) as loading control. This data was developed using the same antibody clone with 68041-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68041-1-Ig (same clone as 68041-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-RPS6KA1 (Thr359/Ser363) antibody (68041-1-Ig, Clone: 2C11D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68041-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68041-1-PBS targets Phospho-RPS6KA1 (Thr359/Ser363) in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ribosomal protein S6 kinase, 90kDa, polypeptide 1
• Calculated Molecular Weight: 735 aa, 83 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC014966
• Gene Symbol: RPS6KA1
• Gene ID (NCBI): 6195
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. RPS6KA1 has some phosphorylation sites including Ser380, Thr359 and Ser363 which are important for kinase activation. (PMID: 16458888, 30813401, 17259979, 10679322)