Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 80108-1-RR (same clone as 80108-1-PBS)
Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.
×
Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 80108-1-RR (same clone as 80108-1-PBS)
WB result of Phospho-RPS6KA1 (Ser380) antibody (80108-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-RPS6KA1 (Ser380) transfected HeLa cells. This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.
WB analysis using 80108-1-RR (same clone as 80108-1-PBS)
Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.
×
Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.
WB analysis using 80108-1-RR (same clone as 80108-1-PBS)
Non-treated Jurkat cells and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.
×
Non-treated Jurkat cells and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.
IHC staining of Jurkat using 80108-1-RR (same clone as 80108-1-PBS)
Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.
IHC staining of Jurkat using 80108-1-RR (same clone as 80108-1-PBS)
Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.
IHC staining of human malignant melanoma using 80108-1-RR (same clone as 80108-1-PBS)
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.
IHC staining of human malignant melanoma using 80108-1-RR (same clone as 80108-1-PBS)
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 80108-1-RR (same clone as 80108-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Phospho-RPS6KA1 (Ser380) antibody (80108-1-RR, Clone: 7F23 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.
FC experiment of Jurkat using 80108-1-RR (same clone as 80108-1-PBS)
1X10^6 Jurkat cells untreated (dashed line) or treated with TPA treated (red) were intracellularly stained with 0.4 ug Anti-Human Phospho-RPS6KA1 (Ser380) (80108-1-RR, Clone:7F23) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.
×
1X10^6 Jurkat cells untreated (dashed line) or treated with TPA treated (red) were intracellularly stained with 0.4 ug Anti-Human Phospho-RPS6KA1 (Ser380) (80108-1-RR, Clone:7F23) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Phospho-RPS6KA1 (Ser380) Recombinant antibody, PBS Only

Phospho-RPS6KA1 (Ser380) Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 80108-1-PBS

Clone No.7F23

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

RPS6KA1, MAPKAP kinase 1a, MAPK-activated protein kinase 1a, MAP kinase-activated protein kinase 1a, HU 1

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

80108-1-PBS targets Phospho-RPS6KA1 (Ser380) in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	ribosomal protein S6 kinase, 90kDa, polypeptide 1
Calculated Molecular Weight	735 aa, 83 kDa
Observed Molecular Weight	83-90 kDa
GenBank Accession Number	BC014966
Gene Symbol	RPS6KA1
Gene ID (NCBI)	6195
RRID	AB_2918865
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q15418
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. It has been reported that the phosphorylation of RPS6KA1 at Ser380 is involved in autophosphorylation by activation of the C-terminal kinase domain. (PMID: 16458888, 30813401, 17259979, 10679322)