Tested Applications
| Positive WB detected in | PDGF treated NIH/3T3 cells, λ phosphatase treated NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29223-1-AP targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ribosomal protein S6 |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC000524 |
| Gene Symbol | RPS6 |
| Gene ID (NCBI) | 6194 |
| RRID | AB_2918253 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62753 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-S6 Ribosomal protein (Ser235/236) antibody 29223-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-S6 Ribosomal protein (Ser235/236) Polyclonal antibody (Cat# 29223-1-AP) has 25 citations across journals including Autophagy, Metabolism, Oncogene, Cell Reports, Free Radical Biology and Medicine, Cell Death Discovery, Journal of Translational Medicine, and others. Research fields span cancer biology (lung, pancreatic, colorectal, liver, esophageal, lymphoma), mTOR signaling, metabolism, autophagy, ferroptosis, bone/osteoarthritis, renal injury, sarcopenia, and vascular biology. Applications cited are primarily WB and IF in human, mouse, and rat.
| Species | Application | Title |
|---|---|---|
Autophagy The ubiquitination of CD274 at MERCs enhances the anti-tumor immunity of cervical cancer. | ||
Metabolism Anti-sarcopenic effects of active vitamin D through modulation of anabolic and catabolic signaling pathways in human skeletal muscle: A randomized controlled trial | ||
Metabolism ALDH2 deficiency aggravates vascular injury-induced restenosis by enhancing vascular smooth muscle cell proliferation through SLC38A2-mediated upregulation of glutamine uptake. | ||
Phytomedicine Eupatilin inhibits non-small cell lung cancer metastasis by suppressing Netrin-1-mediated epithelial-mesenchymal transition. | ||
Aging (Albany NY) RIOK3 promotes mTORC1 activation by facilitating SLC7A2-mediated arginine uptake in pancreatic ductal adenocarcinoma | ||
Cell Death Discov Dapagliflozin regulates chondrocyte homeostasis and protects against osteoarthritis via targets AMPKα and SGLT2. |
Reviews
What customers say
Both reviewers gave 5-star ratings. One used the antibody for Western Blot at 1/1000 dilution on HCT116 cells, praising its high specificity for phospho-RPS6. The other applied it for Immunofluorescence at 1:100 dilution on human brain cortex FFPE tissue, reporting clear detection of mTOR pathway activation in neurons and Balloon Cells. Overall, users highlight strong specificity and reliable performance across different applications and tissue types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Morgane (Verified Customer) (09-15-2025) | Very good anti-phospho-antibody, very specific to RPS6
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Reyes (Verified Customer) (02-14-2025) | pS6 worked perfectly (in green) showing the mTOR activation in my neurons (in red NeuN) and Balloon Cells on human brain FFPE tissue.
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