|Positive WB detected in||PDGF treated NIH/3T3 cells, λ phosphatase treated NIH/3T3 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
29223-1-AP targets Phospho-RPS6 (Ser235/236) in WB, ELISA applications and shows reactivity with Human, Mouse samples.
|Tested Reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||ribosomal protein S6|
|Calculated molecular weight||29 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC000524|
|Gene ID (NCBI)||6194|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.