Phospho-RICTOR (Thr1135) Polyclonal antibody

Phospho-RICTOR (Thr1135) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29117-1-AP

Synonyms

AVO3 homolog, DKFZp686B11164, hAVO3, KIAA1999, mAVO3, Phospho-RICTOR (Thr1135 ), RICTOR

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Validation Data Gallery

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  WB analysis using 29117-1-AP

  Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29117-1-AP (Phospho-RICTOR (Thr1135) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control.

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Tested Applications

• Positive WB detected in: PDGF treated PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29117-1-AP targets Phospho-RICTOR (Thr1135) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: rapamycin-insensitive companion of mTOR
• Calculated Molecular Weight: 192 kDa
• Observed Molecular Weight: 192-200 kDa
• GenBank Accession Number: BC029608
• Gene Symbol: RICTOR
• Gene ID (NCBI): 253260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rictor is the core component of the mammalian target of rapamycin complex 2 (mTORC2), and loss of this protein leads to an immunodeficiency. RICTOR is regulated by phosphorylation at threonine 1135 (Thr1135) by S6K, downstream of PI3K, which inactivates mTORC2 and therefore inhibits AKT phosphorylation at Ser473. (PMID: 26356562, PMID: 28821013)