Phospho-PRAS40 (Thr246) Polyclonal antibody

Phospho-PRAS40 (Thr246) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29072-1-AP

Synonyms

AKT1S1, AKTS1, Lobe, Phospho-PRAS40 (Thr246), PRAS40, Proline rich AKT1 substrate 1

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Validation Data Gallery

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  WB analysis using 29072-1-AP

  Non-treated MCF-7 cells and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 29072-1-AP (Phospho-PRAS40 (Thr246) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 29072-1-AP

  Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29072-1-AP (Phospho-PRAS40 (Thr246) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: IGF-1 treated MCF-7 cells, Calyculin A treated PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29072-1-AP targets Phospho-PRAS40 (Thr246) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: AKT1 substrate 1 (proline-rich)
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC051844
• Gene Symbol: PRAS40
• Gene ID (NCBI): 84335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRAS40 is expressed in a variety of tissues in vivo and has multiple phosphorylation sites, which its activity is closely related to phosphorylation. Studies have shown that PRAS40 is involved in regulating cell growth, cell apoptosis, oxidative stress, autophagy and angiogenesis, as well as various of signalling pathways such as mammalian target of mammalian target rapamycin (mTOR), protein kinase B (PKB/Akt), nuclear factor kappa-B(NF-κB), proto-oncogene serine/threonine-protein kinase PIM-1(PIM1) and pyruvate kinase M2 (PKM2). PGK1 phosphorylated PRAS40 at Threonine 246, which could be inhibited by blocking the interaction. PRAS40 can be phosphorylated by p-AKT at Thr246 site and promote the signal downstream to the mTOR signaling pathway. (PMID: 35058442, PMID: 32855714)