Phospho-POLR2A (Ser7) Polyclonal antibody

Phospho-POLR2A (Ser7) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31139-1-AP

Synonyms

hRPB220, hsRPB1, POLR2, POLR2A, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1, RpIILS, RPO2, RPOL2

---

Validation Data Gallery

• 

  WB analysis using 31139-1-AP

  Phosphatase inhibitor treated HeLa cells, λ phosphatase treated HeLa cells, and non-treated HeLa cells, were subjected to SDS PAGE followed by western blot with 31139-1-AP (Phospho-POLR2A (Ser7) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

---

Tested Applications

• Positive WB detected in: HeLa cells, λ phosphatase treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31139-1-AP targets Phospho-POLR2A (Ser7) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• Calculated Molecular Weight: 217 kDa
• Observed Molecular Weight: 260 kDa
• GenBank Accession Number: NM_000937
• Gene Symbol: POLR2A
• Gene ID (NCBI): 5430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA-directed RNA polymerase II subunit RPB1(POLR2A), is part of the core element of the basic RNA polymerase II transcription mechanism. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for the assembly of factors that regulate transcription initiation, elongation, termination, and mRNA processing.