Phospho-POLR2A (Ser2) Polyclonal antibody

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  WB analysis using 30888-1-AP

  Phosphatase inhibitor treated C2C12 cells, λ phosphatase treated C2C12 cells, and non-treated C2C12 cells were subjected to SDS PAGE followed by western blot with 30888-1-AP (Phospho-POLR2A (Ser2) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

Phospho-POLR2A (Ser2) Polyclonal Antibody for WB, ELISA

Cat No. 30888-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

hRPB220, hsRPB1, POLR2, POLR2A, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1, RpIILS, RPO2, RPOL2

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Tested Applications

• Positive WB detected in: C2C12 cells, λ phosphatase treated C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30888-1-AP targets Phospho-POLR2A (Ser2) in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• Calculated Molecular Weight: 1970 aa, 217 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: BC137231
• Gene Symbol: POLR2A
• Gene ID (NCBI): 5430
• RRID: AB_3669776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P24928
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA-directed RNA polymerase II subunit RPB1 is the catalytic core component of DNA-dependent RNA polymerase II (Pol II). So that the information encoded in DNA can be used, RNA polymerase II makes copies of specific genes. To ensure that RNA polymerase II copies the correct genes at the correct time, a group of regulatory proteins are needed to control its activity. Many of these proteins interact with RNA polymerase II at a region known as the C-terminal domain, or CTD for short. For example, before RNA polymerase can make a full copy of a gene, a small molecule called a phosphate group must first be added to CTD at specific units known as Ser2. (PMID: 31385803)