Phospho-PLCG2 (Tyr1217) Recombinant antibody, PBS Only

• 

  WB analysis using 84456-1-RR (same clone as 84456-1-PBS)

  Non-treated and pervanadate treated Ramos cells were subjected to SDS PAGE followed by western blot with 84456-1-RR (Phospho-PLCG2 (Tyr1217) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84456-1-PBS in a different storage buffer formulation.

Phospho-PLCG2 (Tyr1217) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 84456-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

PLCG2, Phospholipase C IV, Phospholipase C gamma 2, Phosphoinositide phospholipase C-gamma-2, EC:3.1.4.11

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Product Information

84456-1-PBS targets Phospho-PLCG2 (Tyr1217) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: phospholipase C, gamma 2 (phosphatidylinositol-specific)
• Calculated Molecular Weight: 148 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC007565
• Gene Symbol: PLCG2
• Gene ID (NCBI): 5336
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P16885
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Phospholipase C gamma 2 (PLCG2) belongs to the family of phospholipase C-gamma, which is the downstream molecule of the CSF1R. Previous studies have demonstrated that PLCG2 was required for CSF1-induced differentiation of monocytes, microglia, immune, inflammatory responses. PLCG2 phosphorylation leads to an increase in production of the cellular signaling molecules, diacylglycerol (DAG), and inositol 1,4,5 trisphosphate (IP3), which further promotes a wide range of downstream signals, including the phosphorylation of protein kinase C (PKC) family members, such as PKC epsilon (PKCε). (PMID: 32522286)