Phospho-PLCG2 (Tyr1217) Polyclonal antibody

Phospho-PLCG2 (Tyr1217) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29568-1-AP

Synonyms

Phospholipase C gamma 2, Phospholipase C IV, Phospho-PLCG2 (Tyr1217), PLC gamma 2, PLC IV, PLCG2



Tested Applications

Positive WB detected inpervanadate treated Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29568-1-AP targets Phospho-PLCG2 (Tyr1217) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name phospholipase C, gamma 2 (phosphatidylinositol-specific)
Calculated Molecular Weight 148 kDa
Observed Molecular Weight148-150 kDa
GenBank Accession NumberBC007565
Gene Symbol PLCG2
Gene ID (NCBI) 5336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospholipase C gamma 2 (PLCG2) belongs to the family of phospholipase C-gamma, which is the downstream molecule of the CSF1R. Previous studies have demonstrated that PLCG2 was required for CSF1-induced differentiation of monocytes, microglia, immune, inflammatory responses. PLCG2 phosphorylation leads to an increase in production of the cellular signaling molecules, diacylglycerol (DAG), and inositol 1,4,5 trisphosphate (IP3), which further promotes a wide range of downstream signals, including the phosphorylation of protein kinase C (PKC) family members, such as PKC epsilon (PKCε). (PMID: 32522286)

Protocols

Product Specific Protocols
WB protocol for Phospho-PLCG2 (Tyr1217) antibody 29568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols