Phospho-PLCG2 (Tyr1217) Polyclonal antibody
Phospho-PLCG2 (Tyr1217) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 29568-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | pervanadate treated Raji cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
29568-1-AP targets Phospho-PLCG2 (Tyr1217) in WB, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | phospholipase C, gamma 2 (phosphatidylinositol-specific) |
Calculated Molecular Weight | 148 kDa |
Observed Molecular Weight | 148-150 kDa |
GenBank Accession Number | BC007565 |
Gene Symbol | PLCG2 |
Gene ID (NCBI) | 5336 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Phospholipase C gamma 2 (PLCG2) belongs to the family of phospholipase C-gamma, which is the downstream molecule of the CSF1R. Previous studies have demonstrated that PLCG2 was required for CSF1-induced differentiation of monocytes, microglia, immune, inflammatory responses. PLCG2 phosphorylation leads to an increase in production of the cellular signaling molecules, diacylglycerol (DAG), and inositol 1,4,5 trisphosphate (IP3), which further promotes a wide range of downstream signals, including the phosphorylation of protein kinase C (PKC) family members, such as PKC epsilon (PKCε). (PMID: 32522286)
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-PLCG2 (Tyr1217) antibody 29568-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |