Phospho-PLCG2 (Tyr1217) Polyclonal antibody

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  WB analysis of Raji using 29568-1-AP

  Non-treated Raji cells and pervanadate treated Raji cells were subjected to SDS PAGE followed by western blot with 29568-1-AP (Phospho-PLCG2 (Tyr1217) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

Phospho-PLCG2 (Tyr1217) Polyclonal Antibody for WB, ELISA

Cat No. 29568-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

Phospholipase C gamma 2, Phospholipase C IV, Phospho-PLCG2 (Tyr1217), PLC gamma 2, PLC IV, PLCG2

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Tested Applications

• Positive WB detected in: pervanadate treated Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29568-1-AP targets Phospho-PLCG2 (Tyr1217) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phospholipase C, gamma 2 (phosphatidylinositol-specific)
• Calculated Molecular Weight: 148 kDa
• Observed Molecular Weight: 148-150 kDa
• GenBank Accession Number: BC007565
• Gene Symbol: PLCG2
• Gene ID (NCBI): 5336
• RRID: AB_3669689
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16885
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospholipase C gamma 2 (PLCG2) belongs to the family of phospholipase C-gamma, which is the downstream molecule of the CSF1R. Previous studies have demonstrated that PLCG2 was required for CSF1-induced differentiation of monocytes, microglia, immune, inflammatory responses. PLCG2 phosphorylation leads to an increase in production of the cellular signaling molecules, diacylglycerol (DAG), and inositol 1,4,5 trisphosphate (IP3), which further promotes a wide range of downstream signals, including the phosphorylation of protein kinase C (PKC) family members, such as PKC epsilon (PKCε). (PMID: 32522286)