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Phospho-PLCG1 (Ser1248) Polyclonal antibody, PBS Only

Phospho-PLCG1 (Ser1248) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29676-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-PLCG1 (Ser1248) Polyclonal antibody (29676-1-AP)

    WB analysis using 29676-1-AP (same clone as 29676-1-PBS)

    Non-treated and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 29676-1-AP (Phospho-PLCG1 (Ser1248) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29676-1-PBS in a different storage buffer formulation.

Product Information

29676-1-PBS targets Phospho-PLCG1 (Ser1248) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name phospholipase C, gamma 1
Observed Molecular Weight145 kDa
GenBank Accession NumberBC144136
Gene Symbol PLCG1
Gene ID (NCBI) 5335
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Phosphoinositide phospholipase C-gamma-1 (PLCG1) which belongs to the phosphoinositide-specific phospholipase C (PLC) family, is activated by many extracellular stimuli including hormones, neurotransmitters, and growth factors and modulates several cellular and physiological functions necessary for tumorigeneses such as cell survival, migration, invasion, and angiogenesis by generating inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) via hydrolysis of phosphatidylinositol 4,5-biphosphate (PIP2) (PMID: 9242915). Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (PMID: 2472218). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (PMID: 1708307). It has also been shown that PKA-mediated phosphorylation at Ser1248 is inhibitory to PLCγ1 tyrosine phosphorylation and phospholipase activity in CD3-treated Jurkat cells (PMID: 1370476), suggesting that Ser1248 may be an allosteric regulator of PLCγ1 activity.