Phospho-PLA2G4A (Ser505) Polyclonal antibody, PBS Only

• 

  WB analysis using 28925-1-AP (same clone as 28925-1-PBS)

  Non-treated HeLa and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 28925-1-AP (Phospo-PLA2G4A (Ser505) antibody) at dilution of 1:3000 incubated at 4℃ overnight. This data was developed using the same antibody clone with 28925-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 28925-1-AP (same clone as 28925-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated HeLa cells using Phospho-PLA2G4A (Ser505) antibody (28925-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28925-1-PBS in a different storage buffer formulation.

Phospho-PLA2G4A (Ser505) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 28925-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

28925-1-PBS targets Phospho-PLA2G4A (Ser505) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phospholipase A2, group IVA (cytosolic, calcium-dependent)
• Calculated Molecular Weight: 749 aa, 85 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC114340
• Gene Symbol: PLA2G4A
• Gene ID (NCBI): 5321
• RRID: AB_2918213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P47712
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PLA2G4A, also named as cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme which can promote lipid mediator production. PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. PLA2G4A can be activated at the phosphorylation site of ser505 by MAP kinase. (PMID: 25838312, 8381049, 31238788)