Phospho-PKC Delta (Ser359) Polyclonal antibody

Phospho-PKC Delta (Ser359) Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29562-1-AP

Synonyms

MAY1, Phospho-PKC Delta (Ser359), PKCD, PRKCD, Protein kinase C delta type, protein kinase C, delta

---

Validation Data Gallery

• 

  WB analysis using 29562-1-AP

  Non-treated Jurkat, phosphatase inhibitor treated and λ phosphatase treated Jurkat cells were subjected to SDS PAGE followed by western blot with 29562-1-AP (Phospho-PKC Delta (Ser359) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  IF Staining of A431 using 29562-1-AP

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated A431 cells using Phospho-PKC Delta (Ser359) antibody (29562-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Tested Applications

• Positive WB detected in: λ phosphatase treated Jurkat cells
• Positive IF/ICC detected in: λ phosphatase treated A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29562-1-AP targets Phospho-PKC Delta (Ser359) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein kinase C, delta
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC043350
• Gene Symbol: PKC Delta
• Gene ID (NCBI): 5580
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase C (PKC) was initially identified and characterized as a protein hydrolysis-activated kinase called protein kinase M. It has been established that PKC is a family of at least 12 serine/threonine kinases that is divided into three subfamilies: The classical PKCs (α, β1, β2, and γ), which are activated by diacylglycerol (DAG) and calcium; the novel PKCs (δ, ε, η, and θ) which are activated by DAG; and the atypical PKCs (ζ and λ/ι), which respond to neither DAG nor calcium. PKCδ, unlike other members of the PKC family, is unique in its regulation by tyrosine phosphorylation on multiple sites that determine activation, localization, and substrate specificity. PKCδ is activated by inflammatory mediators involved in the inflammatory response including lipopolysaccharide (LPS), tumor necrosis factor (TNF) and interleukin-1 (IL-1). PKCδ activation requires multi-phosphorylation steps which triggers translocation from the cell cytosol to different subcellular compartments. (PMID: 30095599, PMID: 31323909, PMID: 30917487)

Publications

Species	Application	Title
mouse,rat	WB	Cancers (Basel); Tumor-Associated Fibroblast-Derived Exosomal circDennd1b Promotes Pituitary Adenoma Progression by Modulating the miR-145-5p/ONECUT2 Axis and Activating the MAPK Pathway; Authors - Qian Jiang